![]() ![]() Doing so requires a consistent quality measure that is independent of the microscope type and brand. ![]() Any physical deviation of the microscope has consequences for the reproducibility of the images and is therefore important to assess. This importance is, for example, illustrated by the formation and quick growth of the QUAREP-LiMi initiative. When discussing microscopy results, it is important to reflect on the quality of the used acquisition setup. Bottom: line intensity profiles through center of theoretical (purple, dotted) and measured (yellow) PSF, plots not scaled the same. Top: MIPs of theoretical (left) and a measured (right) widefield PSF. From this notion follows the sampling criterium known as the Nyquist rate.Ī multi-channel image requires a multi-channel PSF for deconvolution as the PSFs often differ between channels. Failing to do so spoils any attempt of doing deconvolution, because deconvolution works on the PSF scale. Notably, deconvolution can re-separate neighboring particles with overlapping PSFs, which is not possible with simple deblurring operations.īecause the PSF is the basic "brick" of which microscopy images are "built", one should record details at least on the scale of the PSF to gather all available information. At the end of the process, the model of the object is an accurate representation of the true object, with improved resolution and signal-to-noise ratio relative to the acquired image. Deconvolution reverses the imaging convolution in an iterative fashion: a model of the object is made and iteratively improved upon by convolving it with the PSF and comparing the result to the actual image. If the PSF is known, it can be used to bring the acquired microscopy image closer to the true object through a deconvolution. ![]()
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